Anti-cancerous and Anti-oxidant activity of Pergularia daemia Inspired Zinc Oxide Nanoparticles against Lung Cancer (A549) Cell Line

Abstract: Background and Objectives: In nanotechnology, biosynthesis of nanoparticles has gained considerable attention. Development synthesis plant based metallic with improved properties less undesirable effects have many advantages over the conventional methods. addition to performing their intended function, these phyto antioxidant functionalized can protect against oxidative damage. Pergularia daemia is a therapeutic that been explored identified excellent pharmacological activity, but reported. this study, Zinc Oxide (ZnO) were manufactured from aqueous extract; main objective was evaluate anticancer on A549 cancer cell line. Materials Methods: Aqueous leaf extract prepared, inspired nanoparticle evaluated by FTIR SEM. The viability cytotoxicity ZnO nanoparticles, at various doses, confirmed Trypan blue MTT assay. property investigated estimating Reactive Oxygen Species, Lipid Peroxidase Nitric estimation BAX PARP gene expression Polymerase Chain Reaction (PCR). Results: A decreasing increasing observed in green synthesized dose. Our data enhances activity scavenging free radicals. Elevated apoptotic protein down regulated synthesize treated cells. Conclusion: Based above findings are effective lines proving anti-cancer activity. Keywords: daemia, Nanoparticles, line, Anti-cancer gene, gene.